Rice glutaredoxin GRXS15 confers broad-spectrum resistance to Xanthomonas oryzae pv. oryzae and Fusarium fujikuroi.
Identifieur interne : 000022 ( Main/Exploration ); précédent : 000021; suivant : 000023Rice glutaredoxin GRXS15 confers broad-spectrum resistance to Xanthomonas oryzae pv. oryzae and Fusarium fujikuroi.
Auteurs : Seungmin Son [Corée du Sud] ; Hyeseon Kim [Corée du Sud] ; Kyong Sil Lee [Corée du Sud] ; Soohong Kim [Corée du Sud] ; Sang Ryeol Park [Corée du Sud]Source :
- Biochemical and biophysical research communications [ 1090-2104 ] ; 2020.
Abstract
Rice is a particularly widely consumed food crop globally, but its yield is seriously damaged by bacterial blight due to Xanthomonas oryzae pv. oryzae (Xoo) and bakanae disease due to Fusarium fujikuroi (F. fujikuroi). However, broad-spectrum resistance (BSR) to both Xoo and F. fujikuroi remains largely elusive. In this study, we showed that rice monothiol glutaredoxin GRXS15 localizes in mitochondria and the nucleus, and its transcription is induced by Xoo. Transgenic rice lines constitutively expressing OsGRXS15 showed enhanced disease resistance to Xoo and F. fujikuroi, while CRISPR/Cas9-based knockout mutants showed reduced resistance compared with the wild-type plants. The transcription of pathogenesis-related (PR) genes was significantly induced in OsGRXS15-expressing plants. The rice transcription factor OsWRKY65 was identified as a binding partner, and it directly interacted with OsGRXS15 in the nucleus. Moreover, we revealed that the interaction of OsGRXS15 and OsWRKY65 results in the upregulation of OsPR1. These results suggested that OsGRXS15 interacts with transcription factors, and it confers BSR through regulating the expression of genes related to pathogen response. This is the first report on the nuclear function associated with the monothiol glutaredoxin GRXS15.
DOI: 10.1016/j.bbrc.2020.10.027
PubMed: 33097183
Affiliations:
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Electronic address: srpark@korea.kr.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>National Institute of Agricultural Sciences, Rural Development Administration, Jeonju, 54874</wicri:regionArea><wicri:noRegion>54874</wicri:noRegion></affiliation></author></analytic><series><title level="j">Biochemical and biophysical research communications</title><idno type="eISSN">1090-2104</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Rice is a particularly widely consumed food crop globally, but its yield is seriously damaged by bacterial blight due to Xanthomonas oryzae pv. oryzae (Xoo) and bakanae disease due to Fusarium fujikuroi (F. fujikuroi). However, broad-spectrum resistance (BSR) to both Xoo and F. fujikuroi remains largely elusive. In this study, we showed that rice monothiol glutaredoxin GRXS15 localizes in mitochondria and the nucleus, and its transcription is induced by Xoo. Transgenic rice lines constitutively expressing OsGRXS15 showed enhanced disease resistance to Xoo and F. fujikuroi, while CRISPR/Cas9-based knockout mutants showed reduced resistance compared with the wild-type plants. The transcription of pathogenesis-related (PR) genes was significantly induced in OsGRXS15-expressing plants. The rice transcription factor OsWRKY65 was identified as a binding partner, and it directly interacted with OsGRXS15 in the nucleus. Moreover, we revealed that the interaction of OsGRXS15 and OsWRKY65 results in the upregulation of OsPR1. These results suggested that OsGRXS15 interacts with transcription factors, and it confers BSR through regulating the expression of genes related to pathogen response. This is the first report on the nuclear function associated with the monothiol glutaredoxin GRXS15.</div></front></TEI><pubmed><MedlineCitation Status="Publisher" Owner="NLM"><PMID Version="1">33097183</PMID><DateRevised><Year>2020</Year><Month>10</Month><Day>24</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1090-2104</ISSN><JournalIssue CitedMedium="Internet"><PubDate><Year>2020</Year><Month>Oct</Month><Day>20</Day></PubDate></JournalIssue><Title>Biochemical and biophysical research communications</Title><ISOAbbreviation>Biochem Biophys Res Commun</ISOAbbreviation></Journal><ArticleTitle>Rice glutaredoxin GRXS15 confers broad-spectrum resistance to Xanthomonas oryzae pv. oryzae and Fusarium fujikuroi.</ArticleTitle><ELocationID EIdType="pii" ValidYN="Y">S0006-291X(20)31942-2</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.bbrc.2020.10.027</ELocationID><Abstract><AbstractText>Rice is a particularly widely consumed food crop globally, but its yield is seriously damaged by bacterial blight due to Xanthomonas oryzae pv. oryzae (Xoo) and bakanae disease due to Fusarium fujikuroi (F. fujikuroi). However, broad-spectrum resistance (BSR) to both Xoo and F. fujikuroi remains largely elusive. In this study, we showed that rice monothiol glutaredoxin GRXS15 localizes in mitochondria and the nucleus, and its transcription is induced by Xoo. Transgenic rice lines constitutively expressing OsGRXS15 showed enhanced disease resistance to Xoo and F. fujikuroi, while CRISPR/Cas9-based knockout mutants showed reduced resistance compared with the wild-type plants. The transcription of pathogenesis-related (PR) genes was significantly induced in OsGRXS15-expressing plants. The rice transcription factor OsWRKY65 was identified as a binding partner, and it directly interacted with OsGRXS15 in the nucleus. Moreover, we revealed that the interaction of OsGRXS15 and OsWRKY65 results in the upregulation of OsPR1. These results suggested that OsGRXS15 interacts with transcription factors, and it confers BSR through regulating the expression of genes related to pathogen response. This is the first report on the nuclear function associated with the monothiol glutaredoxin GRXS15.</AbstractText><CopyrightInformation>Copyright © 2020 Elsevier Inc. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Son</LastName><ForeName>Seungmin</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>National Institute of Agricultural Sciences, Rural Development Administration, Jeonju, 54874, South Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kim</LastName><ForeName>Hyeseon</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>National Institute of Agricultural Sciences, Rural Development Administration, Jeonju, 54874, South Korea; Jeongup-si Agricultural Technology Center, Jeongup, 56141, South Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lee</LastName><ForeName>Kyong Sil</ForeName><Initials>KS</Initials><AffiliationInfo><Affiliation>National Institute of Agricultural Sciences, Rural Development Administration, Jeonju, 54874, South Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kim</LastName><ForeName>Soohong</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>National Institute of Agricultural Sciences, Rural Development Administration, Jeonju, 54874, South Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Park</LastName><ForeName>Sang Ryeol</ForeName><Initials>SR</Initials><AffiliationInfo><Affiliation>National Institute of Agricultural Sciences, Rural Development Administration, Jeonju, 54874, South Korea. Electronic address: srpark@korea.kr.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>10</Month><Day>20</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Biochem Biophys Res Commun</MedlineTA><NlmUniqueID>0372516</NlmUniqueID><ISSNLinking>0006-291X</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Broad-spectrum resistance</Keyword><Keyword MajorTopicYN="N">F. fujikuroi</Keyword><Keyword MajorTopicYN="N">OsGRXS15</Keyword><Keyword MajorTopicYN="N">OsWRKY65</Keyword><Keyword MajorTopicYN="N">Rice</Keyword><Keyword MajorTopicYN="N">Xoo</Keyword></KeywordList><CoiStatement>Declaration of competing interest The authors claim no conflicts of interest.</CoiStatement></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year><Month>10</Month><Day>09</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>10</Month><Day>12</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>10</Month><Day>24</Day><Hour>5</Hour><Minute>26</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>10</Month><Day>25</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>10</Month><Day>25</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>aheadofprint</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">33097183</ArticleId><ArticleId IdType="pii">S0006-291X(20)31942-2</ArticleId><ArticleId IdType="doi">10.1016/j.bbrc.2020.10.027</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Corée du Sud</li></country></list><tree><country name="Corée du Sud"><noRegion><name sortKey="Son, Seungmin" sort="Son, Seungmin" uniqKey="Son S" first="Seungmin" last="Son">Seungmin Son</name></noRegion><name sortKey="Kim, Hyeseon" sort="Kim, Hyeseon" uniqKey="Kim H" first="Hyeseon" last="Kim">Hyeseon Kim</name><name sortKey="Kim, Soohong" sort="Kim, Soohong" uniqKey="Kim S" first="Soohong" last="Kim">Soohong Kim</name><name sortKey="Lee, Kyong Sil" sort="Lee, Kyong Sil" uniqKey="Lee K" first="Kyong Sil" last="Lee">Kyong Sil Lee</name><name sortKey="Park, Sang Ryeol" sort="Park, Sang Ryeol" uniqKey="Park S" first="Sang Ryeol" last="Park">Sang Ryeol Park</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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